崔梦琪¹，罗 璇¹，娄湘琴²，梅英杰¹，牟 丹¹，安迈瑞¹，李 政¹，张贞理^1*

（1.淄博市检验检测计量研究总院，淄博255000；2.淄博市淄川区检验检测中心，淄博255000）

摘要：该文利用微波辅助水解技术提取特殊医学用途配方食品中胆碱，并采用酶比色法进行测定。胆碱受到胆碱氧化酶的作用释放过氧化氢（H2O2），在过氧化物酶的作用下，与苯酚和4-氨基安替比林反应生成有色物质，利用分光光度法进行外标法定量。对标准曲线、水解试剂、微波辅助加热条件进行了优化，并将确定的最佳方法在不同基质的样品中进行了实验验证。选择1.0 mol/L盐酸（HCl）溶液在100 ℃下微波辅助水解样品5 min，测定结果和加标回收率与水浴加热水解的国家标准方法相当，微波辅助水解前处理方法简单、快速，准确度高，适用于批量测定特殊医学用途配方食品中胆碱。

关键词：微波辅助水解；酶比色法；胆碱；特殊医学用途配方食品

中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2024）06-0131-0006

Rapid Determination of Choline in Food for Special Medical Purpose by Enzyme Colorimetry Using Microwave-assisted Hydrolysis

CUI Mengqi¹，LUO Xuan¹，LOU Xiangqin²，MEI Yingjie¹，MOU Dan¹，AN Mairui¹，LI Zheng¹，ZHANG Zhenli¹

（1.Zibo Municipal General Institute of Inspection and Metrology，Zibo 255000，China；2.Inspection and Testing Center in Zichuan District of Zibo，Zibo 255000，China）

Abstract：This paper extracted choline from food for special medical purpose using microwave-assisted hydrolysis, and detected the choline content by enzyme colorimetry. Choline was catalyzed by choline oxidase with subsequent discharge of hydrogen peroxide (H2O2). In the presence of peroxidase, hydrogen peroxide reacted with phenol and 4-aminoantipyrine and colored substances were formed. The external standard method was used for quantitative analysis of choline by spectrophotometry. Standard curve, the hydrolysis-solvent and microwave-assisted hydrolysis heating condition were optimized. The determined optimum method was experimentally verified in the samples of different matrices. Selecting samples with 1.0 mol/L HCl solution for microwave-assisted hydrolysis at 100 ℃ for 5 min, the analysis results and spiked recovery rates agreed quite well with those obtained by the national standard method for water bath heating hydrolysis. The pretreatment method of microwave-assisted hydrolysis is simple, rapid and highly accurate, and it is applicable for batch detection of choline in food for special medical purpose.

Keywords：microwave-assisted hydrolysis；enzyme colorimetry；choline；food for special medical purpose

doi：10.3969/j.issn.1674-506X.2024.06-020